23 Jul Method for obtaining mesenchymal cell of umbilical cord
Abstract
The invention relates to the technical field of biological medicine, and in particular relates to a method for obtaining mesenchymal cell of umnilical cord, and the method comprises the following steps: step1, washing the umbilical cord living tissue under an aseptic condition, and cutting into tissue fragments; and step 2, putting the tissue fragments into a culture dish, adding mixed culture solution with low sugar, and then putting the culture dish into a CO2 incubator for cultivation, wherein the mixed culture solution with low sugar in the culture dish is changed every 3-4 days. According to the method, the cells in the umnilical cord can be removed repeatedly through repeated transmission, and the cells are cultivated repeatedly, so that the purpose of obtaining a large amount of cells is achieved, on the basis of production effect above 100%, the yield of the cells is improved, 4.04*10<10> cells are obtained by cultivating cells of the third generation, and 1.56*10<12> cells are obtained by cultivating cells of the sixth generation, so that sufficient cell resources are provided for clinical tissue engineering and gene therapy.
Year: 2013
Country: CN
Doc No: 103045537