23 Jul METHOD FOR OBTAINING CELL CULTURE WITH INDUCED ANGIOGENIC PHENOTYPE INTENDED FOR TISSUE ENGINEERING IN REGION OF ISCHEMIA
Abstract
FIELD: medicine. ^ SUBSTANCE: invention relates to the field of biotechnology, in particular to genetic and cell engineering, and can be used in medicine. Suggested is method for obtaining cell culture for tissue engineering in region of ischemia, which involves co-cultivation in vitro of stromal cells from subcutaneous adipose tissue (SCAT) with cells of either total or enriched with CD31- or NG2-positive cells of cardiomyocite fraction (CMF), which is caried out at ratio of SCAT/CMF of 1:4 to 4:1 until microscopically determined capillary-like structures appear in culture. Cell culture with angiogenic phenotype induced in process of co-cultivation obtained by suggested method ensures effective local activation of angiogenesis when introduced into ischemised tissue. ^ EFFECT: obtaining cell culture, which ensures effective local activation of angiogenesis when introduced into ischemised tissue. ^ 4 cl, 15 dwg, 7 ex
Year: 2008
Country: RU
Doc No: 2334793