23 Jul Method for manufacturing polysaccharide based tissue engineering macroporous support
Abstract
The invention relates to a method for manufacturing a polysaccharide based tissue engineering macroporous support, comprising the following steps: (1) a polysaccharide derivate is prepared into a monomer aqueous solution with the concentration of 0.02-0.1g/ml, wherein the polysaccharide derivate is combination of a macromonomer A and a macromonomer B which can be crosslinked and are respectively obtained by performing chemical modification on two different natural polysaccharides; and (2) the monomer aqueous solution is precooled to 0-4 DEG C, a catalyst is added, then a reaction solution is rapidly placed in a freezing system to be cooled and crystallized and in situ polymerization is carried out, ice crystals are melted at room temperature after the reaction is completed, and holes are formed after the ice crystals are melted, thus obtaining the spongy polysaccharide based tissue engineering macroporous support. The macroporous tissue engineering support manufactured by the invention has the characteristics of high porosity and good pore canal connectivity.
Year: 2011
Country: CN
Doc No: 102018992