23 Jul Method for manufacturing novel biological cartilage support by using umbilical cord Wharton jelly
Abstract
The invention discloses a method for manufacturing a novel biological cartilage support by using umbilical cord Wharton jelly. The method comprises the following steps of: treating an umbilical cord specimen to manufacture a novel biological cartilage support; performing paraffin embedding on a support material, slicing, performing H ematine (HE) dyeing, and observing a porous structure of the material under a light microscope; slicing the manufactured three-dimensional porous support into slices of 50 micrometers, spraying platinum on the surfaces of the slices, observing under a scanning electron microscope, shooting a scanned photograph by selecting appropriate multiples, and observing the appearance of the composite support material; and performing cell-free treatment on the umbilical cord Wharton jelly by combining a physical method and a chemical method, forming by using a freeze-drying method to manufacture the three-dimensional porous sponge-shaped support, wherein cells are removed completely, and a collagen structure is not damaged. According to the method, the umbilical cord Wharton jelly is subjected to the cell-free treatment by combining the physical method and the chemical method, and is formed by a freeze-drying method to manufacture the three-dimensional porous sponge-shaped support successfully, the cells are removed completely, and the collagen structure is not damaged, so that the three-dimensional porous sponge-shaped support is suitable to be used as a support carrier in cartilage tissue engineering.
Year: 2013
Country: CN
Doc No: 102861358