23 Jul Method for manufacturing artificial soft tissue body carried with vascular net flow channel
Abstract
The invention provides a method for manufacturing an artificial soft tissue body carried with a vascular net flow channel. The method comprises the following steps of: firstly designing a soft tissue scaffold model with a vascular structure, layering the soft tissue scaffold model one by one at equal distance, and manufacturing photomask plates of various layers; then uniformly mixing a cell and a collagen solution, and injecting a photocuring hydrogel and a photoinitiator to obtain a photocuring composite solution; injecting the photocuring composite solution to a work table, covering with the photomask plates, curing the photocuring composite solution by adopting a surface exposure technology, and then carrying out curing accumulation layer by layer to obtain a photocured hydrogel soft tissue scaffold with the vascular structure; planting vascular endothelial cells to the vascular structure of the photocured hydrogel soft tissue scaffold, wherein the vascular endothelial cells are attached to the surface of a vascular channel; carrying out static culture and dynamic culture in vitro to obtain the artificial soft tissue body carried with the vascular net flow channel. The method provided by the invention can solve the problems of survival of cells inside a large tissue engineering soft tissue scaffold, and soft tissue scaffold vessel network manufacturing and vascularization in the large injury repair of a soft tissue.
Year: 2014
Country: CN
Doc No: 104027847