23 Jul Method for maintaining and restoring human hair papilla cell primitiveness and application
Abstract
The invention belongs to the technical field of biology and discloses a method for maintaining and restoring human hair papilla cell primitiveness and application. Cationic liposome mediated gene transfection technology is utilized to guide exogenous hTERT gene into normal human DPC in in-vitro culture, in this way, an immortalized human DPC line DPC-hTERT is built successfully; DPC-hTERT is normal cell basically and has no obvious conversion feature, and biological and functional features of normal human DPC are maintained; an ideal cell model is expected to be provided for deeply studying regulation mechanism of DPC biological function change, and sufficient seed cells are provided for building full-function tissue engineering skin containing accessories like hair follicle so as to maintain and restore human hair papilla cell primitiveness. Whole scalp is treated in batches in a hair papilla cell separation method, and hair papilla cells are separated out, so that the method has remarkable effects of high efficiency and quickness.
Year: 2019
Country: CN
Doc No: 109971701