23 Jul Method for inducing in vitro directed differentiation of stem cells through non-contact coculture
Abstract
The invention relates to the field of stem cell and tissue engineering, and discloses a method for inducing in vitro directed differentiation of stem cells through non-contact coculture. The method comprises the following steps of: embedding inducing cells with function of inducing differentiation in biological microcapsules; and coculturing the microcapsules and the stem cells, so that the stem cells are subjected to directed induction and differentiation through the directed induction function of the inducing cells in the microcapsules. The method is easy to operate; and one or more kinds of dioecious and heterogeneous inducing cells are allowed to be used for promoting the in vitro directed differentiation of the stem cells, namely microcapsule films can make inducing factors secreted by the inducing cells permeate the microcapsules to act on the external stem cells, and the inducing cells and the stem cells can be subjected to imunoisolation and do not contact each other directly to facilitate the separation and harvest of different cells. By the method, in vitro directed induction and differentiation of the stem cells can be realized under the condition of conventional static culture, an effective induction method can be provided for dynamically inducing directed differentiation of the stem cells to form three-dimensional tissues in a biological reactor, and the method has important significance for clinical therapeutic research of damaged tissues.
Year: 2011
Country: CN
Doc No: 102102090