23 Jul Method for inducing embryonic stem cell to be differentiated into corneal endothelial cells and inducing culture medium
Abstract
The invention provides an inducing culture medium for inducing to produce corneal endothelial cells. The inducing culture medium is characterized by taking a DMEM (Dulbecco Modified Eagle Medium)/F12 culture medium as a basic culture medium, and is prepared from the following components: beta-mercaptoethanol of which the molar concentration is 0.05 to 0.15 mmol/L, glutamine of which the molar concentration is 0.05 to 0.15 mmol/L, bFGF (Basic Fibroblast Growth Factors) of which the mass concentration is 4 to 8 ng/ml, NEAA (Non Essential Amino Acid) of which the molar concentration is 0.05 to 0.15 mmol/L, KSR (Knockout Serum Replacement) of which the volume concentration is 18 to 25 percent and RA of which the molar concentration is 0.5 to 2 mu mol/L. The invention also provides a method for inducing an embryonic stem cell to be differentiated into the corneal endothelial cells, wherein the corneal endothelial cells which are obtained through inducing are obvious in morphology rule feathers. The method does not involve a feeder layer, so that other animal origin pollution is avoided, and the biological safety is high; the corneal endothelial cells are used as corneal endothelium seed cells in tissue engineering, so that a qualified material source is provided for clinical corneal endothelium transplantation.
Year: 2017
Country: CN
Doc No: 106754717