23 Jul Method for inducing differentiation seedling of manilagrass callus
Abstract
The invention discloses a method for inducing differentiation seedling of manilagrass callus. The method comprises the following steps of: selecting embryonal callus of manilagrass, arranging embryonal callus of manilagrass in a solid medium in which the content of 6-Benzylaminopurine being 0-0.4mg/L and the content of trans-zeatin being 0-0.4mg/L, and growing into seedlings after four weeks. According to the method for inducing the differentiation seedling of the manilagrass callus, disclosed by the invention, a culture medium containing two hormones, namely 6-Benzylaminopurine and trans-zeatin, callus subculturing for a long term is regenerated and cultivated, the regeneration rate of the manilagrass callus subculturing for a long term is remarkably improved, a problem that the regeneration rate of the manilagrass callus subculturing for many years is low is effectively solved, the tissue culture regeneration system of the manilagrass is further modified, and foundation is established for a cell engineering breeding and gene transformation research; and the method is simple to operate and has high efficiency.
Year: 2012
Country: CN
Doc No: 102630565