23 Jul Method for inducing differentiation of stem cells into islet-like cells
Abstract
The invention relates to the field of biomedicines, in particular to a method for inducing differentiation of stem cells into islet-like cells in vitro and use of islet-like cells. The invention aims to provide a method for obtaining a large amount of islet-like cells in vitro and the use of the islet-like cells differentiated under induction as seed cells in transplantation of islet cells and preparation of microencapsulated stem cell preparation and as an in-vitro pharmacokinetic model in cell medicine screening. For realizing the aim, the invention adopts a technical scheme that: 1) constructing an NRSF interfering carrier; 2) separating, culturing and amplifying stem cells; 3) inducing stem cells to differentiate into islet-like cells by two stages; and 4) identifying islet-like cells. The construction of the method has a very bright application prospect in regenerative medicine taking gene engineering, cell engineering, tissue engineering and the like as a basis and is expected to create huge social and economic benefit.
Year: 2012
Country: CN
Doc No: 102311940