23 Jul Method for inducing differentiated cells to prepare mesenchymal stem cells and combination for regulating targets
Abstract
The invention discloses a method for inducing differentiated cells to prepare mesenchymal stem cells and a combination for regulating targets. The method comprises that the differentiated cells are subjected to directional induction to prepare the mesenchymal stem cells; directional induction comprises inhibition of a TGF-beta signal pathway, inhibition of the activity of PKC, activation of a WNT/beta-catenin signal pathway and activation of a cAMP signal pathway. The differentiated cells are induced to the mesenchymal stem cells by phased regulation of the corresponding signal pathways and/orenzyme activity. The differentiated cells can be reprogramed into the mesenchymal stem cells by using the small-molecular compound combination for phased regulation of corresponding targets, all steps can be subjected to precise quality control, and standardization operation and large-scale production are convenient. The method provided by the invention has wide donor sources, a patient himself can be used as a donor, and the mesenchymal stem cells needed for basic research, clinical treatment or tissue engineering production can be obtained in relatively short time.
Year: 2018
Country: CN
Doc No: 108060126