23 Jul Method for in-vitro construction of tissue engineered nerves
Abstract
The invention provides a method for in-vitro construction of tissue engineered nerves. The method adopting a three-dimensional gravity bioreactor comprises the following steps of: culturing seed cells with nerve conduits under a three-dimensional micro-gravity environment, wherein the culture conditions are as follows: the seed cell suspension and nerve conduits are placed to a rotary culture container which is filled with complete medium, the cell final density is 1*10<6>/ml, the culture is carried out in a CO2 incubator of 37 DEG C, the rotation speed with the first 24 hours is 10rpm, so that the cells sufficiently contact and are adhered with the nerve conduits; adjusting the rotation speed of the three-dimensional micro-gravity bioreactor after 24 hours, so that the nerve conduits are suspended in the culture solution; and stopping the culture after 7-14 days to obtain the tissue engineered nerves. The method disclosed by the invention can be used for ensuring that the seed cells adhered with the nerve conduits are more and more uniformly, so that peripheral nerve regeneration is facilitated; and moreover, schwann cells, which are induced by skin-orientated precursor cells, are closer to the true schwann cells and are more suitable for being used as seed cells of nerve tissue engineering.
Year: 2013
Country: CN
Doc No: 103230623