23 Jul Method for improving proliferation ability and performance of umbilical cord blood OECs (outgrowth endothelial cells)
Abstract
The invention discloses a method for improving proliferation ability and performance of umbilical cord blood OECs (outgrowth endothelial cells). The method comprises the steps as follows: pure umbilical cord blood-derived OECs are obtained by separation from fresh umbilical cord blood and purification, and mixed with umbilical cord-derived mesenchymal stem cells for culture, and a culture medium is a EGM-2BulletKit complete medium containing the fetal bovine serum with the volume fraction of 10%. By the aid of the method, the proliferation efficiency and the vascularization ability of the OECs are improved. After the method is applied, due to the immune down-regulatory effect of the mesenchymal stem cells and the expansion of the total treatment cell amount, the cell loss proportion caused by immune reactions is reduced, the treatment effect is guaranteed, and the application possibility of the umbilical cord blood-derived OECs is increased. The mesenchymal stem cells also have the ability of assisting in maintaining blood vessels, so that the long-term treatment effect is guaranteed. The method is mainly applied to blood vessel repair treatment, tissue engineering and the like.
Year: 2014
Country: CN
Doc No: 104099291