23 Jul Method for improving efficiency of transdifferentiation of adipose-derived stem cells into salivary gland acinus-like cells
Abstract
Salivary gland cells and adipose-derived stem cells are respectively cultured by adopting a digestion method; co-culture is performed on the salivary gland cells and the adipose-derived stem cells with a transwell culture chamber and a 24-well culture plate; the salivary gland cells are inoculated in an inner chamber, and the adipose-derived stem cells are inoculated in an outer chamber; a DMEM (dulbecco’s modified eagle medium) containing fetal calf serum of which the volume fraction is 10 percent is adopted as the culture medium; platelet-rich fibrin leach liquor of which the mass fraction is 0.5 to 5 percent is added to induce the transdifferentiation of the adipose-derived stem cells into the salivary gland acinus-like cells. According to a method disclosed by the invention, the efficiency of the transdifferentiation of the adipose-derived stem cells into the salivary gland acinus-like cells can be effectively improved, and seed cells and a new transdifferentiation method are provided for the tissue engineering technology, regenerative medicine research and application.
Year: 2015
Country: CN
Doc No: 104928239