23 Jul Method for improving efficiency of differentiation of umbilical cord mesenchymal stem cells into vascular endothelial cells
Abstract
The invention belongs to the technical field of biological cells, and in particular, relates to a method for improving in-vitro directional induced differentiation of umbilical cord mesenchymal stem cells into vascular endothelial cells. A basic fibroblast growth factor with the concentration of 20 ng/mL and an epidermal growth factor with the concentration of 10 ng/ml are added into a culture liquid (containing an M199 culture medium, fetal bovine serum and a vascular endothelial growth factor) for directional induced differentiation of the umbilical cord mesenchymal stem cells into the vascular endothelial cells, and about 50-70% of the vascular endothelial cells are obtained; compared with pure vascular endothelial growth factor induction (with the differentiation efficiency of about 30%), the differentiation efficiency is improved obviously; the nature and functions of the cells are identified, the obtained cells are confirmed to be the vascular endothelial cells having mature endothelial cell functions, and important experimental basis data are provided for studies of repair of tissues and organs and studies of tissue engineering graft revascularization.
Year: 2017
Country: CN
Doc No: 106609260