23 Jul Method for immortalizing human periodontal ligament stem cell line by using hTERT (human telomerase reverse transcriptase) lentivirus recombinant
Abstract
The invention relates to a method for immortalizing a human periodontal ligament stem cell line by using an hTERT (human telomerase reverse transcriptase) lentivirus recombinant. The method comprises the following steps: 1, culturing primary human periodontal ligament stem cells (hPDLSC); 2, establishing an immortalized genetic engineering cell line hTERT-PDLSC stably expressing hTERT genes, to be specific, infecting the immortalized genetic engineering cell line with primarily cultured PDLSC by using a lentivirus carrier expressing the hTERT genes under a certain condition, and carrying out resistance screening of puromycin to obtain the immortalized cell line stably expressing the hTERT genes, wherein the service life of the cells is prolonged to 35 generations from 3-8 generations, and thus an immortalized cell model having stable properties, favorable functions and normal phenotype is obtained. The method disclosed by the invention has a beneficial effect on research of periodontal ligament stem cells to periodontal tissue engineering as well as research and development of medicaments for effectively treating periodontitis.
Year: 2015
Country: CN
Doc No: 104928319