23 Jul Method for immortalizing human exfoliated deciduous tooth endodontium stem cell line through hTERT recombinant lentivirus
Abstract
The invention relates to a method for immortalizing a human exfoliated deciduous tooth endodontium stem cell line through hTERT recombinant lentivirus. The method comprises: 1, carrying out primary culture on primary human exfoliated deciduous tooth endodontium stem cells SHED; and 2, constructing immortalized human exfoliated deciduous tooth endodontium stem cells hTERT-SHED stably expressing hTERT gene. According to the present invention, the constructed immortalized genetically engineered cells expressing the hTERT gene is adopted as the effective cell model for researching the biological characteristics of the human exfoliated deciduous tooth endodontium stem cells so as to easily and deeply research the applications of the constructed immortalized genetically engineered cells as the seed cells in the periodontal tissue engineering and the actions of different drugs on the cells and the signal transduction pathways of the related action mechanisms, and the constructed immortalized genetically engineered cells are used to screen the traditional Chinese medicine effective components for effectively treating chronic periodontitis so as to provide the powerful tool for the subsequent research of the periodontal tissue repair and reconstruction and the development of the novel effective drugs.
Year: 2015
Country: CN
Doc No: 105063089