23 Jul Method for high-efficiency somatic embryogenesis and plant regeneration method of camellia plants
Abstract
The invention discloses a method for high-efficiency somatic embryogenesis and plant regeneration of camellia plants, comprising the following steps: excising 3/4 leaves of tissue culture seedlings ofcamellia plant tea tree, camellia oleifera or Camellia azalea and using stem segments with 1/4 leaf as explants, inoculating onto a somatic embryo induction medium, culturing at 24-26 DEG C under illumination to form somatic embryos at stem nodes and petioles; cutting the somatic embryos into slices of 1-5mm thick, tiling the slices onto a somatic embryo multiplication medium, and culturing at 24-26 DEG C under illumination to form new somatic embryos; transferring the somatic embryos onto a plant regeneration medium, and culturing at 24-26 DEG C under illumination to generate plantlets fromthe somatic embryos. By using the tender stem segments of camellia plants as the explants, a high-efficiency somatic embryogenesis and plant regeneration system is established, thereby effectively improving the tissue culture propagation efficiency of camellia plants. The method provides technical support for seedling production and genetic engineering improvement of crops such as tea trees, camellia oleifera, camellia, etc.
Year: 2018
Country: CN
Doc No: 108901856