23 Jul Method for high efficiency extracting collagen by alkali swelling acid enzymolysis
Posted at 13:55h
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Abstract
The alkali swelling and acid enzymolysis process of extracting collagen effectively adopts animal tendon or animal skin tissue as material and includes the steps of pre-treatment, separating and refining, and drying and sterilization. The process features the alkali swelling step in 0.1~2 M NaOH solution and the acid enzymolysis step in 0.03~0.8 M acetic acid solution containing pepsase of 0.1~0.5 concentration after the pre-treatment and before the separating and refining. The process has raised collagen solubility, high efficiency and high yield, and the extracted collagen is un-denatured collagen, and has less multimer and high material utilizing rate. The product is used as rack material for tissue engineering.
Year: 2006
Country: CN
Doc No: 1821421