23 Jul Method for establishing osteoporosis model based on microfluidic technology
Abstract
The invention provides a method for establishing an osteoporosis model based on the microfluidic technology. The method comprises the following steps: manufacturing a material by adopting polydimethylsiloxane (PDMS) and glass as core plates, selecting the mouse embryo osteoblast MC3T3-E1 cell line as basic cells, carrying out osteogenesis differentiation induction on the basic cells, carrying out perfusion treatment of hexadecadrol culture solution (with the concentration of 10 mu mol/L) on the differentiation induced osteoblasts in the core plates for 24h at the speed of 0.1 mul/min by adopting a perfusion device, thus establishing an osteoporosis case simultaneous model; on the other hand, carrying out perfusion treatment of hexadecadrol and lithium chloride medicine (with the concentration of 25 mmol/L) on the differentiation induced osteoblasts in the core plates for 24h at the speed of 0.1 mul/min by adopting the perfusion device, thus establishing an osteoporosis treatment module. With the model established by adopting the method provided by the invention, a potential application platform is provided for the fields of tissue engineering, regenerative medicine and the like, and meanwhile, the operation is simple and flexible, the controllability is strong, the automation degree is high, and the consumed amount of the reagent is small.
Year: 2017
Country: CN
Doc No: 106566801