23 Jul Method for establishing human amniotic fluid stem cell line
Abstract
The invention relates to a method for establishing a human amniotic fluid stem cell line. The method comprises the following steps of: 1) performing isolated culture on primary amniotic fluid stem cells; 2) separating and purifying the primary amniotic fluid stem cells subjected to isolated culture; and 3) performing monoclonal screening and amplification culture on the separated amniotic fluid stem cells so as to establish the amniotic fluid stem cell line. The invention provides a rapid, effective and safe method for establishing the human amniotic fluid stem cell line, can realize long-term amplification of the human amniotic fluid stem cells in vitro, can provide a novel seed cell source for tissue engineering research, and makes the human amniotic fluid stem cells serve as seed cells for clinical application.
Year: 2010
Country: CN
Doc No: 101928694