23 Jul Method for establishing high-efficiency regeneration system by using broccoli microspore embryo as explant
Abstract
The invention discloses a method for establishing a high-efficiency regeneration system by using broccoli microspore embryo as explant, and belongs to the technical field of plant tissue culture. The method comprises two main steps of preparing a culture medium and culturing a broccoli microspore embryo plantlet. According to the method, the microspore embryo is used as the explant, the bud inductivity is as high as 100%, the bud number of each explant is 30-50, after the culture medium is added with 0.5-1.0mg/L of zeatin, the bud inductivity and the induction number are respectively increased by 1.1 times and 3.2 times; and after 4.0-5.0mg/L of LAgNO3 is added, the browning rate is reduced by about 10%. By utilizing the method, the efficiency in broccoli tissue culture is greatly improved, and a new technical way for improving the heredity conversion rate is provided. The method can be popularized and applied in broccoli tissue culture and gene engineering heredity improvement.
Year: 2013
Country: CN
Doc No: 103053423