23 Jul Method for efficiently obtaining beating functional cardiomyocytes from mesenchymal stem cells
Abstract
The invention discloses a method for efficiently obtaining beating functional cardiomyocytes from mesenchymal stem cells. The method comprises the following steps: reprogramming the mesenchymal stem cells into pluripotent stem cells through mRNA reprogramming, and then adding an activin A and a bone morphogenetic protein 4 by adopting a matrix sandwich method to obtain the beating functional cardiomyocytes with differentiation efficiency of more than 80%. The differentiation efficiency of cardiomyocytes can be greatly improved by the method provided by the invention. The in vitro multiplication capacity of the cardiomyocytes is limited in the prior art, so the requirement for establishing the myocardium of tissue engineering in vitro cannot be met. The method provided by the invention can provide important experimental data for the research of cell therapy of ischemic cardiovascular diseases, and has a good clinical application prospect.
Year: 2016
Country: CN
Doc No: 105950548