23 Jul Method for directionally inducing cartilage cells
Abstract
The invention belongs to the technical field of biology and relates to a method for directionally inducing cartilage cells, in particular to the method for directionally inducing cartilage cells by human amniotic epithelial cells. The method comprises the following steps: performing primary culture and subculture on the amniotic epithelial cells through in vitro culture of the human amniotic epithelial cells according to a tissue engineering genomics principle; observing and adjusting a genotype of the amniotic epithelial cells; inducing. regulating and controlling the amniotic epithelial cells by a cell factor (a transforming growth factor beta, TGF-beta) and BMP-7 to split the amniotic epithelial cells into the cartilage cells through directional induction; detecting the cartilage cell property and the cartilage cell activity of the induced and split cells by a molecular biology method; determining a regulating and controlling mechanism for directionally inducing the cartilage cellsby the human amniotic epithelial cells; and obtaining a result which indicates that the human amniotic epithelial cells can be used as a seed source for inducing and splitting the cartilage cells. The method gets materials conveniently and has relatively wide application prospect.
Year: 2010
Country: CN
Doc No: 101748095