23 Jul Method for differentiating osteoplastic cells by oriented inducing chick embryo primordial germ cell
Abstract
The invention discloses the method used to directionally induce chick embryo primordial germ cell to differentiate into bone cell in vitro. It uses the inducer formed by 90% DMEM, 10% FBS, 5.5*10-5 beta-mercaptoethanol, 1*10-7mol/L dexamethasone, 0.01mol/L beta-sodium glycerophosphate, 0.05g/L vitamin C to process induction differentiating culturing in vitro for chick embryo primordial germ to differentiate into bone cell finally. It can build cell model for clinical medicine study, widely apply in tissue engineering field for providing reliable experience data, supply new source for cell substituting therapy and tissue therapy, even organ transplantation. The invention has the advantages of simple technology, convenient operation, strong repeatability, no need for special apparatus, only need to master universal cell separating and culturing operation.
Year: 2007
Country: CN
Doc No: 1935987