23 Jul Method for differentiating human multipotential stem cells into hematopoietic stem cells and culture additive
Abstract
The invention discloses a method for differentiating human multipotential stem cells into hematopoietic stem cells and a culture additive. The invention develops a culture solution capable of greatlyimproving the efficiency of differentiating human multipotential stem cells into hematopoietic stem cells. The invention also provides a novel method for differentiating human multipotential stem cells into hematopoietic stem cells. The hematopoietic stem cells can be obtained by adopting the preparation method provided by the invention, and compared with traditional matrix cell co-culture methodand embryoid body culture method, the doubly positive hematopoietic stem cells capable of expressing CD34 and CD343 can be efficiently obtained through the method, and the chemical components are definite and free of animal-derived components, so that the safety of cell preparation is greatly improved, and the method has the characteristics of low time consumption, high differentiation efficiency,relatively low cost and the like. The artificial hematopoietic stem cells can be produced in large scale by adopting the preparation method provided by the invention, the method is stable in qualityand high in safety, and a large number of cell sources are provided for tissue engineering, research and development of drugs and cell therapy.
Year: 2018
Country: CN
Doc No: 108300695