23 Jul METHOD FOR DETERMINING KINETICS OF POLYMER SCAFFOLD BIODEGRADATION IN VIVO
Abstract
FIELD: biotechnology.SUBSTANCE: to determine the kinetics of polymeric scaffold biodegradation in vivo used in tissue engineering and regenerative medicine for the plastic or replacement of body tissue defects, a model of the cranial bone trauma is created on a laboratory animal, a polymer scaffold implantation is conducted with fluorescent labels and inserted stem cells into the formed defect, and a fluorescent signal is recorded from the implanted scaffold. Scaffold is synthesized by the method of microstereolithography under the action of laser radiation. At the same time, fluorescent centers are formed throughout the entire scaffold. Using fluorescence microscopy, the fluorescence intensity of the polymeric scaffold is recorded at 2, 45, and 90 days of the experiment at the excitation wavelengths of 340, 450, and 572 nm, respectively, and the emission wavelengths of 470, 515, and 629 nm.EFFECT: increasing the accuracy of the determination, reducing the toxic load, and simplifying the procedure.2 dwg, 3 ex
Year: 2017
Country: RU
Doc No: 2634032