23 Jul Method for decellularization of human corneal stroma
Abstract
The invention aims to solve the problem of donor shortage of corneal stroma and overcome defects of a traditional decellularization method, provides a method for decellularization of human corneal stroma, and belongs to the technical field of cornea in tissue engineering. The method comprises the following steps: collecting corneal stroma containing tissue cells to be put in a cleaning solution, and washing in the cleaning solution for 2-3 times and 2-5 minutes each time; sealing the corneal stroma in nitrogen, and storing for 5-7 days; taking out the corneal stroma from nitrogen, washing by the cleaning solution for 2-3 times and 2-5 minutes each time, shaking at constant temperature in the cleaning solution, changing the cleaning solution every 1-2 hours to obtain decellularized corneal stroma. The method can be used for completely decellularization, collagen of corneal stroma cannot be hurt, preparations applied in the whole process are non-toxic, the biological characteristics of the corneal stroma can be furthest protected, and the stroma cells can be effectively removed.
Year: 2015
Country: CN
Doc No: 104338181