23 Jul Method for culturing goat precursor fat cells in vitro
Abstract
The invention belongs to the technical field of cell culture engineering, and relates to a method for culturing goat precursor fat cells in vitro. The method comprises the following steps of: preparing a collagenase I culture medium and a cell culture solution, killing a goat, taking goat precursor intercostal fatty tissues out, quickly soaking the fatty tissues into alcohol, repeatedly flushing the fatty tissues by using sterile normal saline, transferring the fatty tissues to a sterilization phosphate buffer solution (PBS), putting the fatty tissues in a sterile room, rinsing the fatty tissues by using the PBS, removing blood vessels and connective tissues by using ophthalmic scissors to obtain a fatty tissue sample, quickly cutting the sample into small blocks, transferring the small blocks to a centrifugal tube, adding the collagenase I culture medium, mixing uniformly and digesting; and performing filtration by using a cell sieve to remove undigested tissue blocks and fatty cells, transferring the digested cell culture solution to the centrifugal tube, centrifuging, removing the supernate, adding the cell culture solution, cleaning, centrifuging, preparing a cell suspension, inoculating cells to a culture dish, and culturing the cells in an incubator. The method is simple, reliable in principle, controllable in culture, good in culture effect and environment-friendly.
Year: 2013
Country: CN
Doc No: 103060268