23 Jul Method for cultivating transgenic rice based on RNAi binary vector
Abstract
The invention relates to a method for cultivating transgenic rice based on an RNAi binary vector, belonging to the technical field of rice gene engineering. The method comprises the steps of selection and clone of a target fragment, palindromic sequence construction of the target fragment, selection of a clone system and construction of RNAi recombinant plasmid, agrobacterium-mediated transformation of the RNAi recombinant plasmid, agrobacterium-mediated method transformation and rice tissue culture, detection of transgenic rice and stress resistance analysis of transgenic plants. According to the method, the RNAi technology is utilized, the rice Csn5 gene expression is effectively reduced, and the method can be used for cultivating a paddy rice transgenic line with a certain resistance and provides technical support for improvement of crop varieties and molecular breeding application in agriculture.
Year: 2017
Country: CN
Doc No: 107446948