23 Jul Method for constructing tissue engineering skin with physiological function
Abstract
The invention discloses a method for constructing tissue engineering skin with a physiological function. Hair follicle stem cells and hair follicle dermal fibroblasts are used as seed cells of the tissue engineering skin, and a cell gel mixed liquid is dropwise added on a human amniotic membrane stent. The human amniotic membrane stent can effectively prevent the contraction of collagen gel, and the human amniotic membrane and organism tissues have very good compatibility during transplantation. At the beginning of transplantation, the human amniotic membrane stent can absorb some nutrients ofthe organism to provide the nutrition for the hair follicle stem cells and the hair follicle dermal fibroblasts and promote the hair production. In the invention, an acute full-thickness skin defectmodel is made by using Guanzhong dairy goat as an experimental animal, and the constructed tissue engineering skin is auto-transplanted. After 2 months of transplantation, the wound recovers well andthe hair grows. After 5 months of transplantation, the wound recovers well and the hair growth situation is good. After 9 months of transplantation, the wound recovers basically.
Year: 2019
Country: CN
Doc No: 109321513