23 Jul Method for constructing tissue engineering epidermis for detecting irritation of cosmetics on polycarbonate film
Abstract
The invention relates to a method for constructing a tissue engineering epidermis for detecting the irritation of cosmetics on a polycarbonate film. The method is characterized by comprising the following steps: (1) carrying out separated culture on epidermization cells; (2) preparing a lipid mixture solution; and (3) constructing the tissue engineering epidermis on the polycarbonate film. The method has the advantages that the operation is easy and convenient and the cost is low; the method is good for synthesis and large-scale production of a product and the product has a basal layer, a spinous layer, a granular layer, a transparent layer and the cuticle tissue engineering epidermises which are in completely differentiated shapes, and the irritation of the cosmetics can be conveniently detected.
Year: 2014
Country: CN
Doc No: 103884835