23 Jul Method for constructing tissue engineering blood vessel
Abstract
The invention provides a preparation method of vessel acellular matrix diaphragm. The method comprises processes of cutting off the vessel along the longitudinal axis of fresh vessel, removing externa, striking the entoderm off, cutting into rectangular vessel blocks, slicing the vessel blocks into rectangular slices along the plane of the vessel blocks, performing acellular operation, and then conducting freeze-drying. The invention further provides a method for constructing tissue engineering blood vessel, which comprises the following steps: a) preparing a vessel acellular matrix diaphragm according to the method; b) culturing vascular smooth muscle cell, and c) constructing the tissue engineering blood vessel by the sandwich technique. The method has the advantages that with the adoption of the slicing method, the fully acellular intravascular stent which is beneficial to cell migration and proliferation is obtained and meanwhile the tissue engineering blood vessel in optional aperture is constructed; the tissue engineering blood vessel constructed by the sandwich technique can ensure that the seed cells are fully planted into the multi-hole structure of the diaphragm that is beneficial to the extending and proliferation of the cells, and in this way, the obtained tissue engineering blood vessel is excellent in property.
Year: 2012
Country: CN
Doc No: 102697581