23 Jul Method for constructing full-biological tissue engineering blood vessel
Abstract
The invention discloses a method for constructing a full-biological tissue engineering blood vessel. The method is characterized in that an acellular tissue matrix is prepared from a sheep carotid artery; endothelial progenitor cells, smooth muscle progenitor cells and adipose-derived stem cells are respectively used as seed cells for constructing tunica intima vasorum, tunica media vasorum and tunica externa; and dynamic processes of wall shear stress, annular tension and axial tensile stress during construction of the blood vessel are realized by a bioreactor. The optimized acellular tissue matrix and optimized seed cell sources are adopted, and standard 3-D (three-dimensional) stress and dynamic regulation are realized in a construction process, so that the structure, mechanical characteristics and biological activities of the constructed tissue engineering blood vessel are close to those of a natural blood vessel. Accordingly, the method has industrial production and clinical application prospects, and a foundation is laid for the tissue engineering blood vessel to access markets.
Year: 2013
Country: CN
Doc No: 102872478