23 Jul Method for constructing biomimetic vascular net in large-volume tissue engineering tissue organ
Abstract
The invention discloses a method for constructing a biomimetic vascular net in a large-volume tissue engineering tissue organ. Firstly, biological information of a large-volume tissue of a human bodyis collected, and inputted into a computer for high biomimetic modeling; then, material separated printing and material-cell mixed printing output are performed with a hydrogel material and a varietyof amplified cells from the tissue by a high-precision biological 3D printer, wherein a hollow part in blood vessels is printed by a controllable degradable hydrogel; after all the hydrogel materialsare solidified, the controllable degradable hydrogel in the blood vessels are removed by means of specific enzymes or chelating reactions or temperature control or illumination and the like, and the vascular net in the large-volume artificial tissue is constructed. The circulation feeding of the vascular net and gas exchange in the large-volume artificial tissue are achieved by connection of mainblood vessels at two ends of the vascular net and a delivery pipe of a culture solution. The central feeding problem of the large-volume tissue is solved, so as to realize the long-term survival and biological function of the large-volume artificial tissue in vitro.
Year: 2019
Country: CN
Doc No: 110408539