23 Jul Method for building vascularized tissue engineering periosteum
Abstract
The invention provides a method for building a vascularized tissue engineering periosteum. The method is characterized by comprising the following steps: 1, preparing a macromolecular micro/nano-fibermembrane by an electrostatic spinning method; 2, inoculating seed cells to the nano-fiber membrane, performing cultivation in vitro and performing directional induction for a period of time; 3, implanting a seed cell/nano-fiber membrane composite body subjected for in-vitro directional induction for a period of time in vivo, performing in-vivo vascularization for a period of time and then takingout to obtain the tissue engineering periosteum. By the method, the rejection reaction of an autologous tissue can be effectively reduced and the vascularization degree of the tissue engineering periosteum can be greatly increased; furthermore, the tissue engineering periosteum is vascularize in vivo in advance, so that the integration time of a blood vessel at the defect position and a blood vessel of the implanted tissue engineering periosteum can be shortened, the healing process of the bone defect is shortened, and the method has a very wide application prospect in the fields of wound healing and tissue engineering.
Year: 2018
Country: CN
Doc No: 107648669