23 Jul Method for building rat islet epithelioid stem cell line
Abstract
The invention relates to a method for building a rat islet epithelioid stem cell line, and belongs to the field of animal cell (tissue) engineering. Collagenase digests pancreatic tissue of an adult rat, pancreas islet is manually chosen, RPMI1640 is cultivated by serum, rat islet primary epithelioid cells are adhered to the wall to grow, a cloning ring is screened, the epithelioid cells are purified, and trypsin digestive juice digests and goes down to the future generation, so as to build the cell line. The rat islet epithelioid stem cell line built by adopting the method has the characteristics that the cell attachment forms polygon epithelioid, is in cloned growth, and is a normal diploid cell. An octamer transcription factor 4, a paired gene cassette 4, a paired gene cassette 6, a neurogenic factor 3 and a nidogen are expressed at the protein level, and the rat islet epithelioid stem cell line has multi-differentiating potential and is free of tumorigenicity.
Year: 2014
Country: CN
Doc No: 103881963