23 Jul Method for building human corneal stromal cell line
Abstract
The invention discloses a method for building human corneal stromal cell line and particularly relates to a method for preparing a human corneal stromal carrier bracket of tissue engineering by utilizing seawater fish collagen. The method comprises the steps of fully dissolving non-enzymatic hydrolysis codfish skin collagen lyophilized powder in hydrochloric acid, centrifuging to remove sediment, subpackaging supernatant into holes of a culture plate, freeze drying, adding 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide/N-hydroxysuccinimide cross-linking agent containing chondroitin sulfate to carry out crosslinking, stabilizing the solution by alkaline Na2HPO4 (disodium hydrogen phosphate) solution, cleaning by 2M of NaCl (sodium chloride), 1M of NaCl (sodium chloride) and distilled water, and freeze drying. The method disclosed by the invention has scientific and rational process; the prepared carrier bracket can be applied to volume production, meets heavy demand of large-scale reconstruction of artificial corneal stroma of tissue engineering on the carrier bracket and creates conditions for recovery sight of blind patients suffering from corneal stroma diseases through clinical corneal transplantation, and the carrier bracket preparation and application in in-vitro reconstruction and clinical treatment of the artificial corneal stroma of the tissue engineering are low in cost.
Year: 2012
Country: CN
Doc No: 102703375