23 Jul Method for analyzing cartilage tissue
Abstract
The invention discloses a method for analyzing a cartilage tissue and belongs to the technical field of cartilage tissue engineering. The method comprises the following steps: performing an autofluorescent scanning experiment on a cartilage matrix of a cartilage tissue in an animal model by utilizing a two-photon confocal microscope and determining the autofluorescent excitation condition of the cartilage matrix; under the conditions, performing full-thickness scanning on a cartilage tissue sample by adopting a three-dimensional scanning mode including an x axis, a y axis and a z axis which are perpendicular to one another and utilizing the two-photon confocal microscope, exciting cartilage matrix autofluorescence on different sub layers in the cartilage tissue sample and obtaining cartilage matrix autofluorescent images on the different sub layers; performing quantitative analysis on the cartilage tissue sample according to the autofluorescent images; reconstructing a three-dimensional image of the cartilage matrix in the cartilage tissue sample by utilizing the cartilage matrix autofluorescent images on the different sub layers and performing qualitative analysis on the cartilage tissue sample. According to the method, not only is complete and accurate cartilage information obtained, but also the cartilage tissue sample can be utilized for multiple times.
Year: 2015
Country: CN
Doc No: 104568880