23 Jul Method for adjusting and controlling tissue blood vessel branches through tension force based on 3D printing frame control
Abstract
The invention relates to the technical field of bioengineering, and provides a method for adjusting and controlling tissue blood vessel branches through tension force based on 3D printing frame control. The method comprises the steps that a PCL injector is loaded and transferred onto a first printing frame of a 3D biological printing machine; a gelatin bracket material is added into a second injector and transferred onto a second printing frame of the printing machine; a mixture of fibrous protein gel and cells is added into a third injector and transferred onto a third printing frame of the printing machine; software and the PCL injector are adjusted; a PCL frame is built through a 3D biological printing platform, and a groove is formed in the PCL frame; the gelatin bracket material is printed and filled into the PCL frame, the mixture of the fibrous protein gel and the cells is printed and fixed to the PCL frame to form a strip, and then, the strip suspends in a culture medium; forming of the blood vessel branches is controlled through the tension force generated through fibrous protein contraction and cell tension, accordingly, a pre-vascularized tissue engineering organization or organ can be built, and the problem that the tissue engineering organization or organ is lack of blood is solved.
Year: 2019
Country: CN
Doc No: 110253876