23 Jul Method for acquiring regeneration plant by Camellia chekiangoleosa Hu anther culture
Abstract
The invention belongs to the field of plant cell engineering, and particularly relates to a method for acquiring a regeneration plant by Camellia chekiangoleosa Hu anther culture. The method includesthe steps: low-temperature pretreatment of Camellia chekiangoleosa Hu anthers; callus tissue induction culture; differentiation culture; rooting culture. Callus tissue induction culture is implementedby the aid of a dark culture mode, a callus tissue induction culture medium used by callus tissue induction culture comprises a hormone combination, anti-browning agents and 30-80g/L sucrose, and thehormone combination includes a combination of 2, 4-D, KT and TDZ or a combination of 2, 4-D, 6-BA and TDZ. According to the method, the callus induction rate, the differentiation rate and the rootingrate of regeneration culture of the Camellia chekiangoleosa Hu anthers can be remarkably increased.
Year: 2019
Country: CN
Doc No: 110604060