23 Jul Manufacturing method of gradient tissue engineering scaffold
Abstract
The invention provides a manufacturing method of a gradient tissue engineering scaffold. The manufacturing method comprises the following steps: printing a biological high molecular material and hydrogel into three types of grids by utilizing a low-temperature deposition 3D (Three Dimensional) printer, so as to obtain a hierarchical structure which comprises a tangential grid corresponding to superficial-layer tangential fibers, a uniform grid corresponding to middle-layer transition fibers and a normal grid corresponding to bottom-layer normal fibers; forming a grid bracket, wherein the grid bracket has the mechanical strength characteristics that a compression resisting force is gradually increased from shallow to deep and an anti-shearing force is gradually reduced from shallow to deep; after printing, carrying out freeze drying on the grid bracket for a first time; adding a dECM (decellularized Extracellular Matrix) solution into pores of the formed grid bracket; carrying out freeze drying for a second time and removing a solvent component in the dECM solution, so as to obtain the composite gradient tissue engineering scaffold which is close to a normal cartilage directional micro-tissue. According to the manufacturing method, one-step molding of the gradient composite scaffold can be realized and a bionic effect is remarkably optimized; quantitative mechanical strength optimization can be carried out very well.
Year: 2017
Country: CN
Doc No: 106693065