23 Jul Maize pollen tissue specific promoter and expression vector thereof
Abstract
The invention discloses a maize pollen tissue specific promoter and an expression vector thereof, and the promoter is 2222bp long, comprises a sequence under the (400) 1 item in a sequence table and is used for replacing a CaMV35S promoter on the plant expression vector pCAMBIA1301 so as to construct the new plant expression vector named p-ZMP5. By means of the obtainment of the promoter, conditions for utilizing genetic engineering to obtain a plant male sterile line and a restoring line can be created, the basis for genetic engineering research is provided and the foundation for specific expression of exogenous genes in pollen is provided, wherein the exogenous genes can be coding genes for killing insect toxin (targeting insects which take the pollen as feed), the genes which can enhance or modify the nutritional value of the pollen and the like. In addition, by interfering with PAB5 genes through RNAi (ribonucleic acid interference), the prevention of the generation of the pollen can be applied in street trees, thereby having a certain alleviating role and the like for pollen allergy and having important theoretical and practical significance.
Year: 2011
Country: CN
Doc No: 102010864