23 Jul LOW-TEMPERATURE PRESERVATION SOLUTION FOR CELLS, SODIUM ALGINATE GEL, CELL GEL, PREPARATION METHOD, USE METHOD AND APPLICATION THEREOF
Abstract
The present invention relates to the technical field of preservation, regeneration and repair of cells, and disclosed thereby is a low-temperature preservation solution for cells, mainly prepared from the following components in percent by mass: 0.8%-1.5% of mannitol, 2%-3% of human serum albumin, 3%-7% of low-molecular dextran, 0.1-0.3% of glutathione, 0.5%-2% of epigallocatechin gallate, 3%-5% of adenosine, 0.5%-1% of glucose, 0.8% of NaCl, 0.2% of KCl, 0.1% of CaCl2 and the balance of water. Also provided by the present invention is a sodium alginate gel, comprising sodium alginate, calcium chloride, hyaluronic acid and the low-temperature preservation solution for cells, and also provided thereby are a cell gel and a preparation method therefor as well as the application of the low-temperature preservation solution for cells, the sodium alginate gel and the cell gel. The present invention has the advantages of the low-temperature preservation solution for cells being able to maintain cell activity at 2-8°C for a long duration, the sodium alginate gel being able to be used as a temporary short-term preservation material for cells from various sources and brackets for cell tissue engineering, and the cell gel may be used as a medical wet wound dressing and may be used for repairing soft tissues, refractory-healing wound skins and mucosal wound injuries.
Year: 2020
Country: WO
Doc No: 2020062282