23 Jul Isolation in vitro and amplification method for adult rat heart microvascular endothelial cell
Abstract
The invention relates to a method for separating and expanding cardiac microvascular endothelial cells of adult rat vitro, which provides an ideal cell model for the correlational studies of cardiac endothelial function and a complementary source of vascular neogenesis treatment and tissue engineering cells after myocardial infarction, and lays foundation for deeply studying the clinical treatment of myocardial infarction, angiocarpy endothelial dysfunction, etc. The method adopts the technical proposal that (1) by utilizing the biological characteristics of the cardiac microvascular endothelial cells, the cardiac microvascular endothelial cells of the adult rat are separated and enriched by adopting a method of enzymic digestion, and are expanded by utilizing the self-renewing and proliferation characteristics of the endothelial cells, thus obtaining a great deal of cardiac microvascular endothelial cells; (2) the separated cells are separated and purified vitro; (3) the separated cells are purified and expanded vitro.
Year: 2009
Country: CN
Doc No: 101463341