23 Jul Isolation, cloning and expression pattern identification of sheep hair follicle specific expression promoter fragment
Abstract
The invention belongs to the technical field of animal genetic engineering and particularly relates to the isolation, cloning and expression pattern identification of a sheep hair follicle specific expression promoter fragment. A promoter with hair follicle tissue expression specificity is isolated from, cloned and identified in a sheep genome. The nucleotide sequence of the promoter is shown as SEQ ID NO:10. A eukaryotic expression vector sKAP11.1p-GFP is constructed through the sheep hair follicle specific expression promoter fragment obtained by cloning; a green fluorescent report gene ZsGeen1 is inserted behind the promoter. The constructed eukaryotic expression vector sKAP11.1p-GFP is integrated into the genome of a transgenic mouse through a pronucleus microinjection method. At the individual level, the fact that the promoter has expression activity in mammals and shows hair follicle tissue specificity is verified. The promoter can be applied to improvement on the wool producing capacity and hair properties of animals.
Year: 2013
Country: CN
Doc No: 103421782