23 Jul Isolation and expression pattern identification of brown planthopper infestation inducible rice promoter zone
Abstract
The present invention belongs to the technical field of plant genetic engineering, and in particular to isolation and expression pattern identification of a brown planthopper infestation inducible rice promoter. The invention is as below: screening to obtain a gene with specific expression in rice green tissue and expression level in up-regulation by the brown planthopper infestation; isolating a promoter zone of the gene from rice genome by a PCR method, naming the promoter as PTG3 with a nucleotide sequence shown as SEQ ID NO: 1; constructing a fusion expression gene by using PTG3 with a GUS reporter gene, and transforming a rice Zhonghua 11 by an Agrobacterium-mediated method; and conducting GUS tissue chemical staining and quantitative RT-PCR detection on the obtained transgenic rice seedling before and after inoculation of brown planthopper. The invention determines that PTG3 promoter can significantly increase the expression level of the downstream gene under the brown planthopper infestation conditions, and the characteristic has potential application value in rice insect-resistant gene engineering breeding.
Year: 2016
Country: CN
Doc No: 105861501