23 Jul Isolated culture method for enameloblast
Abstract
The invention relates to an isolated culture method for enameloblast, and particularly relates to a method for carrying out isolated culture on high-purity enameloblast through the combination of a method for separating dental germ tissues under a stereoscopic microscope and an enzyme digestion method. The method comprises the following steps: after mouse dental germs are separated under a stereoscopic microscope, carrying out primary culture on enameloblast by using a monoclonal method, carrying out purification by using culture solution type replacement and different digestion methods, observing cell morphology and growth conditions under an inverted microscope, and detecting the situations of cellular expressed amelogenin, amelogenin and cytokeratin 14 by using an immumofluorescence method. The growth of enameloblast subjected to primary culture is good, mesenchymal cells are significantly reduced after purification, and epithelial cells are subjected to lamellar growth so as to express keratin, amelogenin and amelogenin. The characteristics of in-vitro-cultured human epithelial enameloblast can be kept for a long time. The establishment of an in-vitro culture method for human dental epithelial cells provides a reliable seed cell source for the research on dental tissue engineering.
Year: 2013
Country: CN
Doc No: 103255101