23 Jul Iris ensata thunb mesophyll protoplast and preparation method thereof
Abstract
The invention discloses an iris ensata thunb mesophyll protoplast and a preparation method thereof. Through pretreatment and plasmolysis of an iris ensata thunb material, and through combined enzymolysis of an iris ensata thunb mesophyll tissue by using cellulase, macerozyme and pectinase, preparation of the iris ensata thunb protoplast is effectively achieved, a technical bottleneck that the irisensata thunb protoplast is difficultly dissociated is overcome, and the efficient preparation method of the iris ensata thunb mesophyll protoplast is effectively established. An effective operating platform is provided for achieving rapid genetic transformation of the iris ensata thumb and transient gene expression research, and a technical support is provided for iris ensata thunb protoplast culture, protoplast fusion, protogenetic genetic transformation, transient gene expression research and the like for establishment of an iris ensata thunb cell engineering technological system.
Year: 2019
Country: CN
Doc No: 109370976