23 Jul Injectable cardiac muscular tissue engineering product and preparation method thereof
Abstract
The invention discloses an injectable cardiac muscular tissue engineering product based on a polysaccharide-polypeptide micro-sphere support and a preparation method thereof, which belong to the technical field of tissue engineering products. The cardiac muscular tissue engineering product comprises cardiac muscle cells and chitosan-polypeptide and carrageenan micro-spheres. The preparation method comprises the following steps of: preparing a chitosan-polypeptide cation micro-sphere and a carrageenan anion micro-sphere respectively by a micro-emulsion method; forming an injectable porous micro-sphere support in a static self-assembly mode; then planting the cardiac muscle cells on the surface of the chitosan-polypeptide micro-sphere; and mixing the chitosan-polypeptide micro-sphere with the carrageenan micro-sphere to form a compound of the injectable porous micro-sphere support and the cells. The micro-sphere support is able to regulate and control a myocardial infarction micro-environment, has a spatial structure which contributes to adhesion and growth of the cardiac muscle cells, facilitates transfer of nutrients and metabolites, and is a novel support material for cardiac muscular tissue engineering.
Year: 2011
Country: CN
Doc No: 102133433